4OUU
anti-MT1-MMP monoclonal antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 66.140, 52.600, 127.500 |
| Unit cell angles | 90.00, 103.68, 90.00 |
Refinement procedure
| Resolution | 52.220 - 2.600 |
| R-factor | 0.19933 |
| Rwork | 0.197 |
| R-free | 0.25271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.612 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.112 | 0.525 |
| Number of reflections | 26041 | |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Potassium Formate 20% PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
