4OUR

Crystal structure of Arabidopsis thaliana phytochrome B photosensory module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97896
Spacegroup name	P 41 21 2
Unit cell lengths	127.502, 127.502, 300.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.565 - 3.400
R-factor	0.2439
Rwork	0.242
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vea
RMSD bond length	0.002
RMSD bond angle	0.570
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.590
High resolution limit [Å]	3.400	5.400	3.400
Number of reflections	35082
Completeness [%]	100.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	278	magnesium sulfate, polyethylene glycol 3340, glycerol, bistris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K