4OUM
Crystal structure of human Caprin-2 C1q domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.499, 77.499, 53.451 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.372 - 1.491 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.21620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 1O91 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.159 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 19420 | |
| <I/σ(I)> | 29.3 | 9.2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 291 | 100mM sodium citrate pH5.6, 12% 2-propanol, 11% PEG4000, VAPOR DIFFUSION, temperature 291K |
