4OUL

Crystal structure of human Caprin-2 C1q domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	P 1
Unit cell lengths	48.353, 48.342, 80.884
Unit cell angles	89.51, 87.24, 71.74

Refinement procedure

Resolution	45.900 - 1.949
R-factor	0.2055
Rwork	0.203
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.907	1.980
High resolution limit [Å]	1.949	1.949
Number of reflections	88743
<I/σ(I)>	24.3	2.5
Completeness [%]	90.0	96.7
Redundancy		2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.6	291	15-20% PEG8000, 0.2M calcium acetate, 0.1M carcodylate pH6.6., VAPOR DIFFUSION, temperature 291K