4OUH

Crystal structure of the FP domain of Human PI31 Proteasome Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	58.830, 97.670, 108.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.176 - 2.000
R-factor	0.1887
Rwork	0.186
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.877
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.176
High resolution limit [Å]	2.000
Number of reflections	42700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	PEG 8000, NaAc, KBr, pH 5.0, vapor diffusion, hanging drop, temperature 298K