4OU7

Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	46.408, 46.689, 54.392
Unit cell angles	87.33, 86.01, 70.20

Refinement procedure

Resolution	38.020 - 2.830
R-factor	0.18611
Rwork	0.183
R-free	0.23827
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.137
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.020
High resolution limit [Å]	2.830
Number of reflections	9917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP