4OU0

Crystal Structure of RPA32C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 65
Unit cell lengths	69.556, 69.556, 23.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.1988
Rwork	0.197
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpu
RMSD bond length	0.003
RMSD bond angle	0.804
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.077	0.357
Number of reflections	12921
<I/σ(I)>	14.46	4.51
Completeness [%]	98.4	89.03
Redundancy	7.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	294	50 mM Sodium Acetate, 20% PEG 3350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K