4OTV
Crystal structure of in cellulo Operophtera brumata CPV18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9686 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.040, 103.040, 103.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.539 - 1.700 |
R-factor | 0.1246 |
Rwork | 0.122 |
R-free | 0.17920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.142 |
Data reduction software | FastDP |
Data scaling software | Blend |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.860 | |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 18161 | |
Completeness [%] | 90.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 301 | Protein crystallized naturally in the cytoplasm, pH 7.5, temperature 301K |