4OTM
Crystal structure of the C-terminal domain from yeast GCN2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.00587 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.126, 118.896, 46.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.138 - 1.950 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.27480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.071 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.040 | 0.025 | 0.260 |
Number of reflections | 18888 | ||
<I/σ(I)> | 23.6 | ||
Completeness [%] | 94.4 | 80.9 | 81.9 |
Redundancy | 3.9 | 4.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris, 21-28% (w/v) polyethylene glycol 2000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |