4OSN
Crystal structure of engineered HCMV glycoprotein B Domain II
Experimental procedure
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 89.922, 89.922, 75.654 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.030 - 1.760 |
R-factor | 0.20337 |
Rwork | 0.202 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.375 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.030 | 1.810 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 18070 | |
Completeness [%] | 98.0 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292.15 | 1.5M Lithium Sulfate, 0.1M Na-HEPES salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |