4OSD
Dimer of a C-terminal fragment of phage T4 gp5 beta-helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.288, 73.786, 111.602 |
| Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
| Resolution | 59.870 - 1.960 |
| R-factor | 0.22229 |
| Rwork | 0.219 |
| R-free | 0.27854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 4JJ2 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.700 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.094 | 0.347 |
| Number of reflections | 116357 | |
| <I/σ(I)> | 6.8 | 2.52 |
| Completeness [%] | 96.9 | 96.3 |
| Redundancy | 2.4 | 2.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 22% PEG 4000, 200mM Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
