4ORG
Crystal structure of human Fab CAP256-VRC26.04, a potent V1V2-directed HIV-1 neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 68.023, 85.471, 103.274 |
Unit cell angles | 97.90, 107.72, 91.67 |
Refinement procedure
Resolution | 35.629 - 3.121 |
R-factor | 0.2755 |
Rwork | 0.275 |
R-free | 0.28860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ocw |
RMSD bond length | 0.003 |
RMSD bond angle | 0.726 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 97.204 | 3.260 | 3.200 |
High resolution limit [Å] | 3.121 | 3.200 | 3.121 |
Rmerge | 0.139 | 0.380 | 0.391 |
Number of reflections | 36109 | ||
<I/σ(I)> | 5.7 | 1.9 | 1.84 |
Completeness [%] | 95.9 | 83.8 | 79.6 |
Redundancy | 1.9 | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 14% PEG3350, 25% isopropanol, 0.1 M Tris, pH 8.0, cryoprotectant: 20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |