4OQW
Crystal structure of mCardinal far-red fluorescent protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.200, 136.700, 167.600 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 47.562 - 2.210 |
R-factor | 0.2249 |
Rwork | 0.223 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.861 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.562 | 47.560 | 2.270 |
High resolution limit [Å] | 2.210 | 9.880 | 2.210 |
Rmerge | 0.131 | 0.020 | 0.822 |
Number of reflections | 113638 | 1227 | 8239 |
<I/σ(I)> | 5.5 | 19.57 | 1.18 |
Completeness [%] | 95.0 | 87.8 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 300 | 0.1 M Tris pH 7.6 - 8.0, 0.2 M CaCl2, 10 mM TCEP, 22 - 25% PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 300K |