4OQC

Urate OXIDASE CO-CRYSTALLIZED WITH AZIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-29
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	78.770, 94.850, 104.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.300
R-factor	0.188
Rwork	0.188
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3l8w
RMSD bond length	0.030
RMSD bond angle	0.076
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.077	0.223
Number of reflections	89456
<I/σ(I)>	22.7	3.2
Completeness [%]	93.3	94.8
Redundancy	4.3	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	PROTEIN AT 20 MG/ML IN 50 MM TRIS-ACETATE BUFFER, 0.3 M SODIUM AZIDE, PH 8.5 PRECIPITATING AGENT: PEG8000 18% IN TRIS BUFFER, BATCH METHOD, temperature 291K