4OQ4
Crystal Structure of E18A Human DJ-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.682, 74.682, 74.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.392 - 1.490 |
| R-factor | 0.1194 |
| Rwork | 0.118 |
| R-free | 0.13870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.085 | 0.634 |
| Number of reflections | 39842 | |
| <I/σ(I)> | 21.5 | 2.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.3-1.6 M sodium citrate, 50 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
