4OPH
X-ray structure of full-length H6N6 NS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 101.690, 101.690, 119.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.974 - 3.158 |
| R-factor | 0.2329 |
| Rwork | 0.230 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2GX9 AND 3F5T |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.522 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.979 | |
| High resolution limit [Å] | 3.158 | 3.158 |
| Rmerge | 0.136 | 0.933 |
| Number of reflections | 6698 | |
| <I/σ(I)> | 12.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.15 | 400 mM magnesium formate, 15% methanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
