4OOV
Crystal structure of P domain from norovirus strain Farmington Hills 2004
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.950, 90.120, 109.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.219 - 1.530 |
| R-factor | 0.1461 |
| Rwork | 0.145 |
| R-free | 0.17090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.219 | 1.540 | |
| High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
| Rmerge | 0.074 | 0.028 | 0.560 |
| Number of reflections | 99095 | 1250 | 7077 |
| <I/σ(I)> | 14.21 | 46.22 | 2.27 |
| Completeness [%] | 99.2 | 97.9 | 97 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Magnesium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
