4OOK
Third Metal bound M.tuberculosis methionine aminopeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Detector technology | IMAGE PLATE |
Collection date | 2014-01-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.221, 48.136, 56.284 |
Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
Resolution | 19.810 - 1.900 |
R-factor | 0.17831 |
Rwork | 0.175 |
R-free | 0.23072 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.857 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.810 |
High resolution limit [Å] | 1.900 |
Number of reflections | 20897 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 27% PEG 2000, BisTris, Oxidized beta-mercaptoethanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |