4OOJ

Crystal structure of the N-terminal domain of the Legionella pneumophila protein SidC at 2.4A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 32
Unit cell lengths	119.055, 119.055, 201.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.900 - 2.400
R-factor	0.2031
Rwork	0.200
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	partial model after phasing and building in AutoSol
RMSD bond length	0.019
RMSD bond angle	1.669
Data scaling software	XSCALE
Phasing software	PHENIX (- AutoSol & PHASER - MR)
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.101	0.579
Number of reflections	124784
<I/σ(I)>	19.03	2.87
Completeness [%]	99.8	99.8
Redundancy	28.65	28.32

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M sodium cacodylate, 0.2M magnesium chloride, 10% PEG 1000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
2	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M sodium citrate, 0.2M sodium acetate, 10% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K