4OOG

Crystal structure of yeast RNase III (Rnt1p) complexed with the product of dsRNA processing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	157.972, 183.804, 61.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.719 - 2.500
R-factor	0.2168
Rwork	0.216
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3RV0 3rv1 1yyw 2nug 1o0w 3n3w 3o2r 3c4t AND 1T4O
RMSD bond length	0.010
RMSD bond angle	0.777
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.650
High resolution limit [Å]	2.500	7.380	2.500
Rmerge	0.066	0.025	0.784
Number of reflections	31418	1329	4870
<I/σ(I)>	14.45	44.12	1.82
Completeness [%]	98.7	98.2	97.5
Redundancy	4.7	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	25% PEG1000, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K