4ONX

2.8 Angstrom Crystal Structure of Sensor Domain of Histidine Kinase from Clostridium perfringens.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	73.903, 82.739, 73.954
Unit cell angles	90.00, 105.92, 90.00

Refinement procedure

Resolution	29.510 - 2.800
R-factor	0.19993
Rwork	0.197
R-free	0.24984
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.506
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.077	0.612
Number of reflections	21394
<I/σ(I)>	31.7	2.7
Completeness [%]	100.0	100
Redundancy	5.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	295	Protein: 7.5 mG/mL, 0.25 M Sodium chloride, 0.01 M Tris-HCL buffer pH 8.3; Screen: Trap96 (B9), 0.2M Ammonium sulfate, 4% (w/v) Sugar; Cryo: 1:1, screen : 50% Sucrose., VAPOR DIFFUSION, SITTING DROP, temperature 295K