4ONR
Crystal structure of Borrelia burgdorferi decorin-binding protein DbpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-13 |
Detector | SBC-3 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 43 |
Unit cell lengths | 49.524, 49.524, 57.943 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.770 - 1.600 |
R-factor | 0.1711 |
Rwork | 0.169 |
R-free | 0.20100 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.135 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.800 | 22.800 | 1.640 |
High resolution limit [Å] | 1.600 | 3.950 | 1.600 |
Rmerge | 0.050 | 0.631 | |
Number of reflections | 18358 | ||
<I/σ(I)> | 33.9 | 1.7 | |
Completeness [%] | 98.8 | 97.8 | 87.9 |
Redundancy | 7.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES, 0.05 M NaCl, 30% PEG 3000, 10% (w/v) ethylene glycol, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1 M CHES, 0.05 M NaCl, 30% PEG 3000, 10% (w/v) ethylene glycol, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |