4ON0
Crystal Structure of NolR from Sinorhizobium fredii in complex with oligo AA DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 |
Unit cell lengths | 131.306, 131.306, 68.548 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.380 - 3.000 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4omy |
RMSD bond length | 0.005 |
RMSD bond angle | 0.861 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.400 |
High resolution limit [Å] | 3.000 |
Number of reflections | 23470 |
<I/σ(I)> | 19.6 |
Completeness [%] | 89.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 20% PEG-3350, 0.1 M sodium citrate/citric acid, and 0.2 M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |