4OM9

X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	77.758, 95.926, 164.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.460 - 2.300
R-factor	0.2224
Rwork	0.220
R-free	0.26280
Starting model (for MR)	3sze
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1439))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.460
High resolution limit [Å]	2.300
Number of reflections	55340
Completeness [%]	99.6
Redundancy	25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	13 mg/ml protein in 50 mM Tris-HCL, 5 mM EDTA, pH 6.5 and 0.3 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K