4OM9
X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.758, 95.926, 164.869 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.460 - 2.300 |
R-factor | 0.2224 |
Rwork | 0.220 |
R-free | 0.26280 |
Starting model (for MR) | 3sze |
RMSD bond length | 0.008 |
RMSD bond angle | 1.143 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1439)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.460 |
High resolution limit [Å] | 2.300 |
Number of reflections | 55340 |
Completeness [%] | 99.6 |
Redundancy | 25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 13 mg/ml protein in 50 mM Tris-HCL, 5 mM EDTA, pH 6.5 and 0.3 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |