4OM3

Crystal structure of human TLE1 Q-domain residues 20-156

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Wavelength(s)	1.03
Spacegroup name	P 21 21 2
Unit cell lengths	49.687, 89.678, 130.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.104 - 2.855
R-factor	0.2369
Rwork	0.234
R-free	0.29760
Structure solution method	MIR
RMSD bond length	0.004
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.800
Rmerge	0.150
Number of reflections	11004
<I/σ(I)>	7.3
Completeness [%]	100.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M HEPES pH 7.0 or 7.5, 15% Tacsimate pH 7.0, 10% PEG 3350, 10% 2-methyl, 2,4-pentanediol (MPD) and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1M HEPES pH 7.0 or 7.5, 15% Tacsimate pH 7.0, 10% PEG 3350, 10% 2-methyl, 2,4-pentanediol (MPD) and 100mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K