4OM2

Crystal structure of TLE1 N-terminal Q-domain residues 1-156

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	CCD
Detector	MAR CCD 130 mm
Wavelength(s)	1.03
Spacegroup name	P 21 21 2
Unit cell lengths	49.586, 94.908, 130.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.686 - 4.000
R-factor	0.2939
Rwork	0.287
R-free	0.34740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.783
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	76.000
High resolution limit [Å]	4.000
Rmerge	0.116
Number of reflections	8169
<I/σ(I)>	4.9
Completeness [%]	100.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	200mM sodium thiocyanate, 15% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K