4OLN
Ancestral Steroid Receptor 1 in complex with estrogen response element DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.184, 36.425, 90.939 |
Unit cell angles | 90.00, 121.63, 90.00 |
Refinement procedure
Resolution | 41.372 - 1.700 |
R-factor | 0.1764 |
Rwork | 0.175 |
R-free | 0.20590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.965 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1593) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.102 | 0.111 | 0.296 |
Number of reflections | 36436 | ||
<I/σ(I)> | 15.6 | ||
Completeness [%] | 83.3 | 99.5 | 32.7 |
Redundancy | 3.6 | 4.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 12% PEG3350, 100 mM ammonium acetate, 100 mM Bis-Tris, pH 5.5, microseeded with a 1:100 dilution of crushed crystals of the same protein:DNA construct grown using higher concentration PEG3350 and 75 mM ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |