4OLK
The CHAP domain of LysGH15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 113.359, 113.359, 178.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.849 - 2.694 |
| R-factor | 0.1775 |
| Rwork | 0.174 |
| R-free | 0.20390 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.690 |
| Number of reflections | 18004 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M Bis-Tris-propane, pH 7.5, 3.8M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
