4OKV

Crystal structure of anopheline anti-platelet protein with Fab antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	298
Detector technology	CCD
Collection date	2011-07-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	93.810, 99.426, 166.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.624 - 1.802
R-factor	0.2198
Rwork	0.218
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.134
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.093	0.038	0.433
Number of reflections	135319
<I/σ(I)>	9.6
Completeness [%]	94.5	97.9	80.8
Redundancy	3.9	5.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES, 15% PEG 20000, pH 7.0, vapor diffusion, hanging drop, temperature 298K