4OKR
Structures of Toxoplasma gondii MIC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-30 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.791, 148.052, 41.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.300 - 2.601 |
R-factor | 0.2323 |
Rwork | 0.230 |
R-free | 0.27550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.734 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1565)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.300 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.268 | |
Number of reflections | 15634 | |
Completeness [%] | 99.3 | 63.4 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 293 | 0.1 M sodium acetate, 0.2 M (NH4)2SO4, 25% PEG 4000, pH 4.6, VAPOR DIFFUSION, temperature 293K |