4OKF
RNase S in complex with an artificial peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.800, 43.800, 96.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.590 - 1.540 |
| R-factor | 0.17148 |
| Rwork | 0.171 |
| R-free | 0.20955 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2rns |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.7.0029) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.600 |
| High resolution limit [Å] | 1.540 |
| Rmerge | 0.053 |
| Number of reflections | 16225 |
| <I/σ(I)> | 30.62 |
| Completeness [%] | 97.5 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 292 | 0.1 M sodium citrate, 2.4 M ammoniumsulfate, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
