4OKF
RNase S in complex with an artificial peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-10-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 43.800, 43.800, 96.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.590 - 1.540 |
R-factor | 0.17148 |
Rwork | 0.171 |
R-free | 0.20955 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2rns |
RMSD bond length | 0.019 |
RMSD bond angle | 1.972 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.7.0029) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.600 |
High resolution limit [Å] | 1.540 |
Rmerge | 0.053 |
Number of reflections | 16225 |
<I/σ(I)> | 30.62 |
Completeness [%] | 97.5 |
Redundancy | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 292 | 0.1 M sodium citrate, 2.4 M ammoniumsulfate, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K |