4OKC
Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 22.960, 42.360, 56.990 |
| Unit cell angles | 84.24, 85.91, 84.81 |
Refinement procedure
| Resolution | 35.360 - 2.250 |
| R-factor | 0.22475 |
| Rwork | 0.222 |
| R-free | 0.26458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xd5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.103 | 0.300 |
| Number of reflections | 9500 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 94.2 | 92.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.2M tri-sodium citrate, 0.1M Na HEPES, 6% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
