4OJO
Crystal Structure of Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120 in Complex with Lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.411, 152.881, 171.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.000 - 2.000 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.21400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | SeMet Tailspike structure |
RMSD bond length | 0.007 |
RMSD bond angle | 1.411 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.404 |
Number of reflections | 211941 | |
<I/σ(I)> | 11.3 | 2.2 |
Completeness [%] | 97.0 | 93.3 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris pH 7.0-7.6, 20% PEG-1000. Soaked in mother liquor containing 37% saturated lactose, VAPOR DIFFUSION, HANGING DROP, temperature 298K |