4OJL
Crystal Structure of Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120 in Complex with Glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.448, 152.442, 171.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.800 - 2.000 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.21100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | SeMet Tailspike structure |
RMSD bond length | 0.007 |
RMSD bond angle | 1.427 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.800 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.107 | 0.439 |
Number of reflections | 209831 | |
<I/σ(I)> | 13 | 2.9 |
Completeness [%] | 96.2 | 92.2 |
Redundancy | 6.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris pH 7.0-7.6, 20% PEG-1000. Soaked in 37% saturated glucose mother liquor, VAPOR DIFFUSION, HANGING DROP, temperature 298K |