4OJJ

Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator (Space group : P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	200
Detector technology	CCD
Collection date	2010-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	36.430, 173.540, 175.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.860 - 2.320
R-factor	0.2336
Rwork	0.230
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.166
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.410
High resolution limit [Å]	2.300	2.300
Rmerge	0.054	0.560
Number of reflections	49604
<I/σ(I)>	15.7	2.4
Completeness [%]	99.6	98.2
Redundancy		3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG4K, 50mM Na Citrate, 5% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K