4OJE
Crystal structure of a C-terminally truncated trimeric ectodomain of the C. elegans fusion protein EFF-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2011-03-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00150 |
| Spacegroup name | P 63 |
| Unit cell lengths | 106.886, 106.886, 135.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.720 - 2.570 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22090 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 27936 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 5.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 11% PEG 3000, 16mM Na2HPO4, 84mM KH2PO4, 0.25% DDM, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
