4OJ6
Crystal Structure of a Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120; Se-Met Protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97945, 0.97961, 0.94945, 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.299, 153.086, 171.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.851 - 1.800 |
R-factor | 0.1881 |
Rwork | 0.187 |
R-free | 0.20950 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.138 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.087 | 0.500 |
Number of reflections | 297461 | |
<I/σ(I)> | 13.3 | 2.1 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 6.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris pH 7.0-7.6, 20% PEG-1000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |