4OJ5
Crystal Structure of a Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.621, 147.823, 170.631 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.438 - 1.800 |
R-factor | 0.1867 |
Rwork | 0.187 |
R-free | 0.20330 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | SeMet Tailspike protein structure |
RMSD bond length | 0.007 |
RMSD bond angle | 1.423 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.900 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.094 | 0.510 |
Number of reflections | 287155 | |
<I/σ(I)> | 11.9 | 2.2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 6.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris pH 7.0-7.6, 20% PEG-1000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |