4OIZ
Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.510, 80.860, 100.940 |
| Unit cell angles | 90.00, 130.69, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.400 |
| R-factor | 0.2443 |
| Rwork | 0.242 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native structure |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.786 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.156 | 0.430 |
| Number of reflections | 10344 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 98.4 | 98.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.0M ammonium citrate tribasic, 0.1M bis-tris propane, 60mM methyl-alpha-mannose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
