4OIJ
X-ray crystal structure of racemic non-glycosylated chemokine Ser-CCL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.480, 48.860, 51.050 |
| Unit cell angles | 109.46, 106.84, 109.00 |
Refinement procedure
| Resolution | 18.860 - 2.000 |
| R-factor | 0.2363 |
| Rwork | 0.234 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.000 | 2.150 |
| Rmerge | 0.078 | 0.519 |
| Number of reflections | 16601 | |
| <I/σ(I)> | 9.4 | 3.2 |
| Completeness [%] | 75.7 | 8.4 |
| Redundancy | 2.9 | 0.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 292 | 2 M Ammonium Sulfate, 0.1 M citric acid, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
