4OII
West Nile Virus NS1 in complex with neutralizing 22NS1 antibody Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-17 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.869, 49.546, 130.075 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 49.467 - 3.000 |
| R-factor | 0.2163 |
| Rwork | 0.214 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.682 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.124 | 0.057 | 0.698 |
| Number of reflections | 28187 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 9 mg/mL in 8% PEG 8K, 0.1 M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
