4OI9
Crystal Structure of ICAM-5 D1-D4 ectodomain fragment, Space Group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.590, 46.910, 95.790 |
| Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
| Resolution | 22.421 - 2.500 |
| R-factor | 0.2513 |
| Rwork | 0.248 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.931 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.131 |
| Number of reflections | 23117 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 294 | 100 mM Tris-HCl 8.5, 10% PEG4000, pH 5.6, VAPOR DIFFUSION, temperature 294K |
