4OI0

bound to ssRNA dinucleotide GC, ADP, AlF4-, and Mg2+(transition state, data set I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Detector technology	IMAGE PLATE
Collection date	2010-08-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	100.710, 100.710, 40.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.660 - 2.200
R-factor	0.22938
Rwork	0.227
R-free	0.27970
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	22553
Completeness [%]	96.9	83.7
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM Na2HPO4/KH2PO4 200 mM NaCl 15 mM MgCl2 90 mM sarcosine 25% (w/v) PEG 1000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K