4OHQ
Crystal structure of chloroplast triose phosphate isomerase from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 100.920, 100.920, 221.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.880 - 2.150 |
R-factor | 0.1722 |
Rwork | 0.170 |
R-free | 0.21170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.145 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX (dev_1593) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.885 | 28.880 | 2.220 |
High resolution limit [Å] | 2.150 | 8.860 | 2.150 |
Rmerge | 0.091 | 0.044 | 0.796 |
Total number of observations | 6816 | 43071 | |
Number of reflections | 37207 | ||
<I/σ(I)> | 17 | 31.4 | 4.6 |
Completeness [%] | 99.9 | 95 | 100 |
Redundancy | 13.9 | 11 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M HEPES Sodium, 1.4 M Sodium citrate tribasic dihydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |