4OH3
Crystal structure of a nitrate transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.800, 188.465, 262.848 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.909 - 3.250 |
R-factor | 0.2384 |
Rwork | 0.235 |
R-free | 0.30670 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.454 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.250 | 3.200 |
Rmerge | 0.070 | 0.910 |
Number of reflections | 34573 | |
<I/σ(I)> | 1.2 | 1.4 |
Completeness [%] | 99.0 | 93.5 |
Redundancy | 5.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1M sodium acetate 30% PEG300 3% MPD, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |