4OGS
Crystal structure of GFP S205A/T203V at 2.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.609, 86.609, 119.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.210 |
R-factor | 0.18738 |
Rwork | 0.181 |
R-free | 0.30630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.813 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.220 |
Rmerge | 0.098 |
Number of reflections | 27588 |
<I/σ(I)> | 31 |
Completeness [%] | 99.9 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Crystals were produced using 1 uL protein (26 mg/ml in 0.1 M imidazole, pH 7.8) mixed with 1 uL well solution, Crystallization screens varied from 22% to 32% (w:v) polyethylene glycol monomethyl ether (PEG) 2000 and 0.05M to 0.2M KBr at room temperature, for a range of pH values near neutrality, VAPOR DIFFUSION, HANGING DROP, temperature 298K |