4OG5
Human menin with bound inhibitor MIV-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Detector technology | CCD |
Collection date | 2012-08-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.663, 80.131, 124.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.140 - 1.630 |
R-factor | 0.16425 |
Rwork | 0.163 |
R-free | 0.19396 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gpq |
RMSD bond length | 0.017 |
RMSD bond angle | 1.822 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.140 |
High resolution limit [Å] | 1.630 |
Number of reflections | 61076 |
<I/σ(I)> | 23.47 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 283 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 and 25% w/v PEG 3,350. This solution was mixed 1:1 with 2.5mg/mL protein in 50mM Tris-HCl (pH 8.0), 50mM NaCl, and 1mM TCEP. Prior to data collection, crystals were transferred into a cryo-solution containing 20% PEG550 MME and flash-frozen in liquid nitrogen, VAPOR DIFFUSION, SITTING DROP, temperature 283K |