4OG4
Human menin with bound inhibitor MIV-3S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2011-10-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.915, 80.207, 124.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.480 - 1.450 |
| R-factor | 0.16295 |
| Rwork | 0.162 |
| R-free | 0.18856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gpq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.480 |
| High resolution limit [Å] | 1.450 |
| Rmerge | 0.087 |
| Number of reflections | 87254 |
| <I/σ(I)> | 36.5 |
| Completeness [%] | 100.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 283 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 and 25% w/v PEG 3,350. This solution was mixed 1:1 with 2.5mg/mL protein in 50mM Tris-HCl (pH 8.0), 50mM NaCl, and 1mM TCEP. Prior to data collection, crystals were transferred into a cryo-solution containing 20% PEG550 MME and flash-frozen in liquid nitrogen, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
