4OG2
The crystal structure of a solute-binding protein (N280D mutant) from Anabaena variabilis ATCC 29413 in complex with leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.326, 101.124, 149.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.498 - 1.099 |
R-factor | 0.1303 |
Rwork | 0.129 |
R-free | 0.14970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nqr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.112 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.500 | 1.120 |
High resolution limit [Å] | 1.099 | 1.100 |
Rmerge | 0.074 | 0.620 |
Number of reflections | 296681 | |
<I/σ(I)> | 25.1 | 1.53 |
Completeness [%] | 98.8 | 91 |
Redundancy | 5.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Magnesium Chloride, 0.1 M Tris:HCl, 30% (v/v) PEG 400, 10mM Leucine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |