4OFZ
Structure of unliganded trehalose-6-phosphate phosphatase from Brugia malayi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 166.940, 166.940, 99.562 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.908 - 3.000 |
R-factor | 0.2213 |
Rwork | 0.216 |
R-free | 0.27320 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.722 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 16791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 15 mg/ml, crystallization buffer: 33% PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol (EG) and 10 mM CoCl2. For cryoprotection, the concentration of EG was slowly increased to 25% by adding a concentrated solution directly to the crystal drop. Crystals were harvested using CryoLoops (Hampton Research), looped through LV CryoOil (MiTeGen), frozen in liquid nitrogen, VAPOR DIFFUSION, HANGING DROP, temperature 290K |